Plasmid_Backbone

Part:BBa_K1678008:Design

Designed by: Prateek Garg   Group: iGEM15_Paris_Bettencourt   (2015-09-17)


A biobrick shuttle plasmid working between Lactobacillus plantarum, and E.coli with erythromycin res


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 15
    Illegal SpeI site found at 2880
    Illegal PstI site found at 2866
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 15
    Illegal SpeI site found at 2880
    Illegal PstI site found at 2866
    Illegal NotI site found at 6
    Illegal NotI site found at 2871
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 15
    Illegal BamHI site found at 1786
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 15
    Illegal SpeI site found at 2880
    Illegal PstI site found at 2866
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 15
    Illegal SpeI site found at 2880
    Illegal PstI site found at 2866
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

A part of pSIP409 was extracted with tails and re-circularized.


Source

It's been made from the plasmid pSIP409

References